Vascular Endothelial Growth Factor or VEGF is a family of proteins which excite vasculo-genesis and angiogenesis. The VEGF family contains 5 members: VEGF-A, VEGF-B, VEGF-C, VEGF-D and PGF (placenta growth factor). Being the very first VEGF family member detected, VEGF-A is often only known as VEGF.
VEGF165 and VEGF121 are predominant isoforms expressed in most cells and cells. VEGF165 is a glycoprotein and contains moderate affinity to heparin. In contrast, VEGF 121 does not bind heparin. VEGF specifically binds tyrosine kinase receptors. You can buy high-quality VEGF ELISA equipment to measure this growth factor.
VEGFR-1 (Flt-1) and also VEGFR-2 (Flk-1) to trigger downstream signaling pathways resulting in endothelial cell survival, proliferation, migration, and vascular permeability. VEGF plays a vital role in normal vascular reproductive and bronchial angiogenesis. On the other hand, VEGF is fundamental to pathological angiogenesis by improving vessel permeability or recruitment endothelial progenitor cells to remote sites of neovascularization.
The Human Vascular Endothelial Growth Factor (Hu VEGF) ELISA quantitates Hu VEGF in human serum, plasma, buffered solution, or cell culture medium. The assay will only comprehend both natural and recombinant Hu VEGF. The Individual VEGF solid-phase sandwich ELISA is intended to assess the total amount of the object bound involving a coordinated antibody pair.
A target-specific antibody was pre-coated from the wells of their provided microplate. Samples, criteria, or controllers are subsequently added within these molds and bind to the immobilized (capture) antibody. VEGF has a number of impacts on vascular endothelium, including the capability to promote endothelial cell viability, mitogenesis, chemotaxis, and vascular permeability.